Search，Order，Antibody,ELISA Kit & Protein

Introduction

l For the quantitative determination of TNF-α concentrations. 

l Detection of species: Fish

l Detection medium: serum, plasma, tissue homogenates, cell culture supernates

Principle of test

The kit is for the quantitative level of TNF-αin the sample, adopt purified Human TNF-αto coat microtiter plate, make solid-phase antibody, then add TNF-αto wells, Combine TNF-αantibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of TNF-αin the samples is then determined by comparing the O.D. of the samples to the standard curve. 

DETECTION RANGE

Detection range of the kit is: 3ng/L -80ng/L