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l   For the quantitative determination of Human Neopterin concentrations.

l   Detection of species: Human

l   Detection medium: serum, plasma, tissue homogenates, cell culture supernates, Saliva

Principle of test

The kit is for the quantitative level of Neopterin in the sample, adopt purified Human Neopterin to coat microtiter plate, make solid-phase antibody, then add Neopterin to wells, Combine Neopterin antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of Neopterin in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Composition of the kit