Human Glutathione Peroxidase (GSH-PX) ELISA KitHuman
serum, plasma, tissue homogenates, cell culture supernates
Principle of test
The kit is for the quantitative level of GSH-PX in the sample, adopt purified Human GSH-PX to coat microtiter plate, make solid-phase antibody, then add GSH-PX to wells, Combine GSH-PX antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of GSH-PX in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Chromogen Solution A(1×6ml)
Chromogen Solution B(1×6ml)
Wash Solution(1×20ml×30 fold)
For Research Use Only!
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