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l   For the quantitative determination of Human G-CSF concentrations.

l   Detection of species: Human

l   Detection medium: serum, plasma, tissue homogenates, cell culture supernates,urine

The kit is for the quantitative level of G-CSF in the sample, adopt purified Human G-CSF to coat microtiter plate, make solid-phase antibody, then add G-CSF to wells, Combine G-CSF antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of G-CSF in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

DETECTION RANGE

Detection range of the kit is: 20ng/L -800ng/L