If you wish to check out in vitro measurement for AIF1 in tissue, cell culture supernatant, serum, plasma and biological fluids, then you can surely try using the Human Allograft inflammatory factor 1 (AIF1) ELISA Kit.

With 96 strip wells, this uses the colorimetric detection method, which has the sensitivity of 11.6 pg/mL.  While reaction testing no interference was noticed between analogues and Allograft Inflammatory Factor 1. The primary reaction of this is on humans and it has a detection range of 31.25-2000 pg/mL.

Components of this kit:

This Human Allograft inflammatory factor 1 (AIF1) ELISA Kit has certain features that are specific to it, for better experiment conditions.

·         Standard Diluent

·         Assay Diluent A

·         Detection Reagent A (green)

·         Detection Reagent B (red)

·         Plate sealer for 96 wells

·         Wash Buffer

·         Assay Diluent B

·         TMB Substrate

With an assay time of 4.5 hours, and sample volume of 100 μL, this kit is extremely useful as colour change detects the change in content.

Sample collection:

The samples are collected from such tissues and plasma, which help in determining the actual content. The temperature range for this varies within -20°C or -80°C in case of serum collection. For cell lysate, centrifuge is to be done at 1500 × g for 10 minutes within a range of 2 - 8°C to remove cellular debris.

After all of this is taken for experimentation, the Human Allograft inflammatory factor 1 (AIF1) ELISA Kit can be used for deriving correct results.