With Human Beta Defensin Beta 2(B-D2) ELISA Kit one can find out research usage in terms of quantification of BD-2 in cultures of cells belonging to humans. The qualified samples for determination of these cells are saliva, tissue fluids and serum.

There are other kits belonging to this zone is available for BD 1, BD 3, NP1, mouse, rat, bovine, monkey, chicken, rat and pig.

Principle used:

In this case, the principle that is used happens to be the binding of human beta Defensin 2, one captured on microtiter wells, while the other is conjugated to biotin. After the coagulation, washing step is done, wherein chromogenic substrate is added.

Specific performance features:

·         In case of human serum, assay of frozen sera ranging from 0 to 770 pg/ml is taken.

·         In case of human saliva, assay of fresh sample 705 pg/ml is taken.

These samples are to be well treated before checking out Human Beta Defensin Beta 2(B-D2) ELISA Kit.

Contents of the kit:

·         Human beta Defensin 2 standard.

·         Sample diluent concentrate.

·         Anti-human beta Defensin 2 Detection antibody concentrate.

·         Wash solution concentrate type

After gathering of these components, there are incubation slots such as first, second and third. After all of this is done, can the final stop step be reached and final conclusions determined.

Hence, with help of this Human Beta Defensin Beta 2(B-D2) ELISA Kit cell cultures can be checked in the correct manner.