The need for Human Pepsin(PP) ELISA Kit arises at a time when there is a need for quantitative determination of human pepsin concentrations in plasma, serum, tissue homogenates and urine. By means of the colorimetric detection method, the pepsin content can be checked with sensitivity being 10 pg/ml.

These kits are to be specifically used for research purposes and not diagnostic procedure.

Detailing of the product:

·         The sensitivity is maintained at 7.81 pg/mL.

·         The assay has specificity and high detection sensitivity for human pepsin.

·         With an assay time limit of 1 - 4.5 h and sample volume of    50 μL, this uses the quantitative sandwich enzyme immunoassay process.

How to collect samples and store them?

The samples for Human Pepsin(PP) ELISA Kit are to be stored in a specific manner.

·         Serum:  A separator tube is used which allows samples to clot for half an hour prior to centrifugation for 15 minutes at 1000g. Samples are to be stored at -20° C after removing serums.

·         Plasma: To be collected using citrate EDTA within 30 minutes of collection, it has to be centrifuged for 15 minutes.

·         Cell culture supernates: Particulates are to be removed by centrifugation and samples to be stored at -20° C.

Assay testing procedure:

·         Precision within assay: Three known concentrated samples were tested twenty times on a single plate.

·         Precision between assay: Three concentrated samples were tested in twenty assays.

Basic details:

The Human Pepsin(PP) ELISA Kit is to be stored at 4 °C/-20 °C. For long term storage it is to be stored at -20°C.

So, you can get a perfect kit for researching on pepsin.