With help of Rat Alpha-fetoprotein (AFP) ELISA Kit the determination of at alpha-fetoprotein (AFP) concentrations can be done in quantitative terms in plasma, tissue homogenates, cell culture supernates, and serum.

With a sensitivity of 3.9 pg/mL and colorimetric detection method, the detection ranges within 15.6-1000 pg/mL. The concerned antigen is stated to be Alpha-fetoprotein.

Contents of this kit:

·         Assay plate (12 × 8 coated Microwells)

·         Biotin-antibody Diluent - 1 × 10mL

·         Stop Solution - 10mL

·         HRP-avid in (100 × concentrate) - 120µL

·         Standard (freeze dried)

·         TMB Substrate

·         Sample Diluent

·         Biotin-antibody (100 × concentrate)

·         Adhesive Strip (for 96 wells)

Thus, with all these components, this Rat Alpha-fetoprotein (AFP) ELISA Kit is strictly used for research purposes by making use of reagents.

Reagent preparation:

The components and samples that have been prepared by collecting and centrifuging plasma, cell supernates and serum is brought to room temperature at (18-25°C).

Next, 5 µL of lysis buffer solution is dispensed into 50 µL s, with a proportion of 1:10 and kept for an hour. After this, it is to be diluted by 10 mL of Wash Solution concentration with 990 mL of distilled solution to finally prepare 1000 mL of Wash Solution.

After this the general assay procedure is followed with general results in regards to quantitative data. However, reagents from different sorts are never to be mixed.

So you can get best result in this regard by choosing Rat Alpha-fetoprotein (AFP) ELISA Kit!