To quantify the level of STAT3 protein phosphorylated at tyrosine residue 705 you can surely make use of Rat STAT3 ELISA Kit. This is specifically suitable for only research purpose regarding detection of mouse, human and rat STAT3 [pY705] from tissue or cell homogenates by making usage of microplate reader and 96 well plates.

The sample type is of specifically adherent cell cultures with storage at 4°C.

Components of this kit:

·         96-Well Cell Culture Clear-Bottom Microplate.

·         HRP-Conjugated Anti-Mouse IgG Antibody

·         100x Anti-STAT3 Antibody (Rabbit Polyclonal)

·         Crystal Violet Solution

·         Adhesive Plate Seals

·         SDS Solution.

·         100x Anti-GAPDH Antibody (Mouse Monoclonal)

·         Quenching Buffer

·         Ready-to-Use Substrate.

Characteristics of performance:

There are certain specific features that are specific to this Rat STAT3 ELISA Kit.

·         Standard curve range: 1.57–100 Units/mL

·         Assay incubation time: 4 hours.

·         Species cross-reactivity: mouse, human, rat

·         Sensitivity: <0.9 Units/mL

·         Sample types: tissue homogenates, cell lysates

·         Sample volume: 100 μL (1:10)

Methodology:

Rat STAT3 ELISA Kit used is sandwich enzyme-linked immunosorbent assay. That monoclonal which is antibody specific for STAT3 is coated on to wells of the microtiter strips. Samples containingSTAT3 [pY705] are then pipetted on to the wells. As the first incubation process happens, STAT3 antigen binds itself to the captured antibody.

During phase of second incubation, antibody becomes a detection antibody by binding itself to the immobilized STAT3 protein that was captured in the initial incubation. The coloured product that finally comes as a residue is directly proportional to the concentration of STAT3 [pY705] present in the actual specimen.

With help of Rat STAT3 ELISA Kit this test can be performed in the best manner.