Glucagon is a specific type of peptide hormone that is produced within the pancreas’s alpha cells. Its primary functions are to increase glucose content within the blood of individuals. In stark comparison to insulin, that helps in reducing glucose content in the body, this helps in maintaining a balance within the body. This Glucagon acts as a general marker in case of low content of glucose in the body. With this peptide, during lower concentrations, stored glycogen in liver is converted to glucose that can be used by the body for important balance maintenance. Also, to raise the Glucagon levels in the body, it is important to have meals that are rich in protein than carbohydrate. In case a person is suffering from diabetes of type 1, then the presence of high level of insulin can actually help in reduction of Glucagon content in the body. This on another level can work to counterbalance insulin levels in the body. As per tests conducted with help of Mouse Glucagon (GC) ELISA Kit, various internal blood regulated secretions are taken into consideration. Starting off from serum, to saliva, especially in case of mice, the level of glucagon context is checked and made sure that there are chances of improvement and balance maintenance in case of further tests.