In Human glutamic acid decarboxylase (GAD) ELISA Kit, there is a proportionate level of GAD usage to make this solution appear strait fast in its duty to cover up microtiter plate. The sample application will help in building antibody in solid phase. There is a compound built up with proper mixture of antibody, enzyme and complex form found with them.

This sample GAD Kit helps in adding GAD to the wells with HRP. When it is applied after wash the color turns into blue. The HRP enzyme is also found to be catalyzed which can be stopped with solution built for this and measuring it to the level of nm at 450.