The sample of Human Protein DJ-1 (PARK7) ELISA Kit will present a proper solution in coating microtiter plate by solid-phase purified antigen and adding PARK7 into the wells. After uniting with HRP and adding antigen to this mixture to create proper complex by antigen, antibody and enzyme.

After applying PARK7 sample Kit, there may appear color changes, blue to be exact and HRP enzyme can be affected with chemical reactions. But this effect can be subsided by a proper solution especially made from sulfuric acid to stop these effects. In the sample Kit these are measured with a process called spectrophotometric very easily and fast.