There is perfect application LKM to the wells in Human anti-liver kidney microsome autoantibody (LKM) ELISA Kit. It has been proved to create a protective coating to microtiter plate and to combine antigen to the HRP. The compound created with antigen, antibody and enzyme in the sample LKM Kit is effective enough to apply after wash.

The TMB substrate solution will change color into blue which has another common appearance as catalyzed process of HRP enzymes. This reaction found in it that can be easily subsided by application of a solution specifically made by sulfuric acid. The color changes can be recognized with a specific process.