Definition of hydroxyproline

Hydroxyproline, also known as cis-4-hydroxy-L-proline.Hydroxyproline is one of the amino acids, usually with a hydroxyl in the fourth position, but sometimes also in the first three. Because there are two asymmetric carbon atoms, there are four kinds of stereoisomers. It contains L-hydroxyproline in animal glue and collagen. There is no D-hydroxyproline in nature. L-Hydroxyproline is a component of the toxic polypeptide phalloidine obtained from Amanita Phalloides. In general, the protein does not have such an amino acid. In the methyl derivatives of hydroxyproline, there are betonicine, turicine (these are found in Stachyssilvatica and betonica, officinalis) and γ-hydroxyhygric acid exist in the skin of Croton gubonga of Croton. In vivo, hydroxyproline is synthesized from proline, but not in the free state, but in the peptide chain is hydroxylated. Its decomposition similar to proline, but with the state of hydroxyl, so as 4-hydroxy-glutamic acid. Reaction with ninhydrin gives a yellow solution.

Collagen is composed of 18 kinds of amino acids, hydroxyproline is only one of these 18 amino acids. But because it is relatively rare in other common protein (such as soy protein), it can be used as a characteristic of one of the amino acids, the content of which is the source of the collagen protein by the decision, but also in the same raw materials, different types Of the collagen hydroxyproline content is not the same.

Human protein with collagen hydroxyproline content of the most, while collagen is composed of connective tissue collagen fibers in the main component. Collagen fibers are widely distributed in the body of various organs, so collagen is the body's most protein, about the total body protein 1/3. The use of Hyp in the highest content of collagen in this feature, through the blood and urine on the determination of Hyp, can understand the catabolism of collagen in the body, can be used as indicators of connective decomposition. Amino acid automatic analyzer is widely used in clinic.

Hydroxyproline traits

Hydroxyproline is a white flaky crystalline or crystalline powder. The unique sweetness in the bitter taste improves the flavor of the fruit juice beverage and the refreshing drink. A special flavor, can be used for Hong raw materials. The temperature of 274 ° C (decomposition). Specific rotation: -59 º (C = 2, H2O),

Refractive index: -59 °(C = 2, H2O),Soluble in water (25 ℃, 36.1%), slightly soluble in ethanol.

The use of hydroxyproline

Flavor enhancer; nutritional fortifier; Mainly used for fruit juice, soft drinks, nutritional drinks; for biochemical reagents.

Hydroxyproline production method

By gelatin, bone glue, casein, soybean skin and other proteins, hydrolysis with hydrochloric acid, nitrosation method after extraction by resin chromatography after resin purification, crystallization. The yield was about 7.0%.

Relationship between Hydroxyproline and Collagen

The source of the raw material determines the collagen protein amino acid composition, in which hydroxyproline content has also been fixed. So hydroxyproline content, with the final product collagen content is not the corresponding.

Collagen is the role of a variety of amino acids on the mutual cooperation, not just a certain amino acids, hydroxyproline although the role of the skin is very good, but not that its higher the effect of the content is good.

In conclusion:

A: hydroxyproline is one of the characteristics of collagen, but not the only active ingredient.

B: hydroxyproline content determined by the source, and the overall collagen content is not a linear relationship

C: Collagen effect by its overall structure to play a role, and denaturation temperature and so on.