Detection range of the kit is: 60 pg/ml -3600 pg/ml
The kit is for the quantitative level of MMP-12 in the sample, adopt purified human
MMP-12 to coat microtiter plate, make solid-phase antibody, then add samples or
standards to wells with a labeled antibody specific to MMP-12, then add labeled HRP to
the well. After washing completely, add TMB substrate solution, TMB substrate
becomes blue color in wells that contains antibody - antigen - enzyme-antibody
complex, reaction is terminated by the addition of a stop solution and the color
change is measured at a wavelength of 450 nm. The concentration of MMP-12 in the
samples is then determined by comparing the O.D. of the samples to the standard
curve.