Detection range of the kit is: 4.5pg/mL - 183pg/mL
The kit is for the quantitative level of MMP-II in the sample, adopt purified human
MMP-II antibody to coat microtiter plate, make solid-phase antibody, then add samples
or standards to wells with a labeled antibody specific to MMP-II, then add labeled HRP
to the well. After washing completely, add TMB substrate solution, TMB substrate
becomes blue color in wells that contains antibody - antigen - enzyme-antibody
complex, reaction is terminated by the addition of a stop solution and the color
change is measured at a wavelength of 450 nm. The concentration of MMP-II in the
samples is then determined by comparing the O.D. of the samples to the standard
curve.