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The kit is for the quantitative level of GHRP-GHrelin in the sample, adopt purified Human GHRP-GHrelin to coat microtiter

plate, make solid-phase antibody, then add GHRP-GHrelin to wells, Combine GHRP-GHrelin antibody with labeled HRP to

form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate

becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change

is measured at a wavelength of 450 nm. The concentration of GHRP-GHrelin in the samples is then determined by

comparing the O.D. of the samples to the standard curve.