Search，Order，Antibody,ELISA Kit & Protein

The kit assay Human LEP level in the sample，use Purified Human LEP antibody to coat microtiter plate wells, make solid-phase antibody, then add LEP to wells, Combined LEP which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LEP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

DETECTION RANGE

Detection range of the kit is: 0.3μg/L -8μg/L