Search，Order，Antibody,ELISA Kit & Protein

The kit is for the quantitative level of HK in the sample, adopt purified Human HK to coat microtiter plate, make solid-phase antibody, then add HK to wells, Combine HK antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of HK in the samples is then determined by comparing the O.D. of the samples to the standard curve.