Search，Order，Antibody,ELISA Kit & Protein

The kit assay human SP-D level in the sample，use Purified human SP-D antibody to coat microtiter plate wells, make solid-phase antibody, then add SP-D to wells, Combined SP-D which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of SP-D in the samples is then determined by comparing the O.D. of the samples to the standard curve.