Search，Order，Antibody,ELISA Kit & Protein

CALCULATION OF RESULT
Test validity: the average of Positive control well≥1.00; the average of Negative control well ≤0.10.
Calculate Critical (CUT OFF): Critical= the average of Negative control well + 0.15.
Negative control: sample OD< Calculate Critical (CUT OFF) is ANA Negative control
Positive control: sample OD≥ Calculate Critical (CUT OFF) is ANA Positive control.
PRINCIPLE OF TEST
The kit is for the qualitative determination of ANA in the sample, adopt purified antigen to coat microtiter plate, make solid-phase antigen, then pipette samples to the wells, with anti- Mouse ANA conjugated Horseradish Peroxidase (HRP). Wash and remove non-combinative antibody and other components. Antibodies specific for the antigen will bind to the pre-coated antigen. After washing completely, add TMB substrate solution and color develops according to the amount of ANA. Reaction is terminated by the addition of a stop solution and the intensity of the color is measured at a wavelength of 450 nm. Compared with the CUTOFF value to judge if ANA exists in the sample or not.