Search，Order，Antibody,ELISA Kit & Protein

The kit is for the quantitative level of LDH in the sample, adopt purified Rat LDH to coat microtiter plate, make solid-phase

antibody, then add LDH to wells, Combine LDH antibody with labeled HRP to form antibody-antigen -enzyme-antibody

complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP

enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a

wavelength of 450 nm. The concentration of LDH in the samples is then determined by comparing the O.D. of the samples

to the standard curve.