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The kit is for the quantitative level of α-SMA in the sample, adopt purified human α-SMA to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to α-SMA, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of α-SMA in the samples is then determined by comparing the O.D. of the samples to the standard curve.