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 For the quantitative determination of Human NGF-βconcentrations.
 Detection of species: Human
 Detection medium: serum, plasma, tissue homogenates, cell culture supernates

The kit is for the quantitative level of NGF-β in the sample, adopt purified Human NGF-β to coat microtiter plate, make solid-phase antibody, then add NGF-β to wells, Combine NGF-β antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of NGF-β in the samples is then determined by comparing the O.D. of the samples to the standard curve.