Search，Order，Antibody,ELISA Kit & Protein

The kit is for the quantitative level of MIF in the sample, adopt purified Human MIF to coat microtiter plate, make solid-phase antibody, then add MIF to wells, Combine MIF antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of MIF in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

DETECTION RANGE

Detection range of the kit is: 3μg/ L-80μg/ L