Search，Order，Antibody,ELISA Kit & Protein

Principle of test

The kit is for the quantitative level of EDN1 in the sample, adopt purified Human EDN1 to coat microtiter plate, make solid-phase antibody, then add EDN1 to wells, Combine EDN1 antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of EDN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Kit Components

Microelisa Stripplate(12well×8strips)

Standard (1×0.5ml)

Standard Diluent(1×1.5ml)

HRP-Conjugate Reagent(1×6ml)

Sample Diluent(1×6ml)

Chromogen Solution A(1×6ml)

Chromogen Solution B(1×6ml)

Stop Solution(1×6ml)

Wash Solution(1×20ml×30 fold)

User manual(1)

Adhesive Strip(2)

USAGE

For Research Use Only!