Search，Order，Antibody,ELISA Kit & Protein

The kit assay Rat Hyp level in the sample，use Purified Rat Hyp antibody to coat microtiter plate wells, make solid-phase antibody, then add Hyp to wells, Combined Hyp antibody which With HRP labeled, become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of Hyp in the samples is then determined by comparing the O.D. of the samples to the standard curve.

DETECTION RANGE

Detection range of the kit is: 50μg/L -1000μg/L

This our product references：

1：Accelerated wound healing and anti-inflammatory effects of physically cross linked polyvinyl alcohol–chitosan hydrogel containing honey bee venom in diabetic rats

Author: Mohamed A. AminIhab T. Abdel-Raheem