Search，Order，Antibody,ELISA Kit & Protein

Detection range of the kit is: 10ng/ml -500ng/ml

 

The kit is for the quantitative level of SPARC in the sample, adopt purified Human SPARC to coat microtiter plate, make

solid-phase antibody, then add SPARC to wells, Combine SPARC antibody with labeled HRP to form antibody-antigen

-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at

HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a

wavelength of 450 nm. The concentration of SPARC in the samples is then determined by comparing the O.D. of the

samples to the standard curve.