Search，Order，Antibody,ELISA Kit & Protein

The kit is for the quantitative level of NGF in the sample, adopt purified Human NGF to coat microtiter plate, make solid-phase antibody, then add NGF to wells, Combine NGF antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of NGF in the samples is then determined by comparing the O.D. of the samples to the standard curve. 

DETECTION RANGE

Detection range of the kit is: 0.7ng/L -15ng/L