Detection range of the kit is: 0ng/L-15ng/L

 

The kit is for the quantitative level of bFGF1 in the sample, adopt purified Human bFGF1

antibody to coat microtiter plate, make solid-phase antibody, then add samples or

standards to wells with a labeled antibody specific to bFGF1, then add labeled HRP to

the well. After washing completely, add TMB substrate solution, TMB substrate

becomes blue color in wells that contains antibody - antigen - enzyme-antibody

complex, reaction is terminated by the addition of a stop solution and the color

change is measured at a wavelength of 450 nm. The concentration of bFGF1 in the

samples is then determined by comparing the O.D. of the samples to the standard

curve.