Search，Order，Antibody,ELISA Kit & Protein

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in

this kit has been pre-coated with an antigen specific to RVF-IgM. Standards or samples

are added to the appropriate Microelisa stripplate wells and combined to the specific

antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for RVF is

added to each Microelisa stripplate well and incubated. Free components are washed

away. The TMB substrate solution is added to each well. Only those wells that contain

RVF-IgM and HRP conjugated RVF antigen will appear blue in color and then turn yellow

after the addition of the stop solution. The optical density (OD) is measured

spectrophotometrically at a wavelength of 450 nm. The presence of RVF-IgM is

determined by comparing with the CUTOFF value.