Human sorbitol dehydrogenase,SDH ELISA Kit
HumanCatalog #:96780
Introduction
l For the quantitative determination of SDH concentrations.
l Detection of species: Human
l Detection medium: serum, plasma, tissue homogenates, cell culture supernates
Principle of test
The kit is for the quantitative level of SDH in the sample, adopt purified Human SDH to coat microtiter plate, make solid-phase antibody, then add SDH to wells, Combine SDH antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of SDH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
DETECTION RANGE
Detection range of the kit is: 3.5μmol/L -120μmol/L
Product No.:96780
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