Search，Order，Antibody,ELISA Kit & Protein

The kit is for the quantitative level of CSF in the sample, adopt purified human CSF to coat microtiter plate, make

solid-phase antibody, then add CSF to wells, Combine CSF antibody with labeled HRP to form antibody-antigen

-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue

color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is

measured at a wavelength of 450 nm. The concentration of CSF in the samples is then determined by comparing

the O.D. of the samples to the standard curve.