Search，Order，Antibody,ELISA Kit & Protein

The kit is for the quantitative level of COL- IV in the sample, adopt purified Human COL- IV to coat microtiter plate, make

solid-phase antibody, then add COL- IV to wells, Combine COL- IV antibody with labeled HRP to form antibody-antigen

-enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color

at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a

wavelength of 450 nm. The concentration of COL- IV in the samples is then determined by comparing the O.D. of the

samples to the standard curve.