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This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated

with an antibody specific to DHEA-S. Standards or samples are added to the appropriate Microelisa stripplate wells and

combined to the specific antibody. Then a Horseradish Peroxidase (HRP)- conjugated antibody specific for DHEA-S is added

to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added

to each well. Only those wells that contain DHEA-S and HRP conjugated DHEA-S antibody will appear blue in color and then

turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a

wavelength of 450 nm. The OD value is proportional to the concentration of DHEA-S. You can calculate the concentration of

DHEA-S in the samples by comparing the OD of the samples to the standard curve.