l For the quantitative determination of Bovine GH concentrations.
l Detection of species: Bovine
l Detection medium: serum, plasma, tissue homogenates, cell culture supernates
Principle of test
The kit is for the quantitative level of GH in the sample, adopt purified Bovine GH to coat microtiter plate, make solid-phase antibody, then add GH to wells, Combine GH antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of GH in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Composition of the kit
Reagent | Quantity |
Microelisa Stripplate | 12well×8strips |
Standard: 5400pg/ml | 1×0.5ml |
Standard Diluent | 1×1.5ml |
HRP-Conjugate Reagent | 1×6ml |
Sample Diluent | 1×6ml |
Chromogen Solution A | 1×6ml |
Chromogen Solution B | 1×6ml |
Stop Solution | 1×6ml |
Wash Solution | 1×20ml×30 fold |
User manual | 1 |
2 |
Product No.:D8569
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