Human Klotho protein(KL) ELISA Kit
Human For the quantitative determination of human KL concentrations.
Detection of species: Human
Detection medium: serum, plasma, tissue homogenates, cell culture supernates,urine
PRINCIPLE OF TEST
The kit is for the quantitative level of KL in the sample, adopt purified human KL to coat microtiter plate, make solid-phase
antibody, then add KL to wells, Combine KL antibody with labeled HRP to form antibody-antigen -enzyme-antibody complex,
after washing completely, add TMB substrate solution, TMB substrate becomes blue color at HRP enzyme-catalyzed,
reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The
concentration of KL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product No.:90281
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