Search，Order，Antibody,ELISA Kit & Protein

The kit is for the quantitative level of FGF-23 in the sample, adopt purified human FGF-23 to coat microtiter plate, make solid-phase antibody, then add samples or standards to wells with a labeled antibody specific to FGF-23, then add labeled HRP to the well. After washing completely, add TMB substrate solution, TMB substrate becomes blue color in wells that contains antibody - antigen - enzyme-antibody complex, reaction is terminated by the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The concentration of FGF-23 in the samples is then determined by comparing the O.D. of the samples to the standard curve.