Human N-acetyl-β-D-glucosaminidase (NAG )ELISA Kit
HumanThe kit is for the quantitative level of NAG in the sample, adopt purified Human NAG to coat microtiter
plate, make solid-phase antibody, then add NAG to wells, Combine NAG antibody with labeled HRP
to form antibody-antigen -enzyme-antibody complex, after washing completely, add TMB substrate
solution, TMB substrate becomes blue color at HRP enzyme-catalyzed, reaction is terminated by
the addition of a stop solution and the color change is measured at a wavelength of 450 nm. The
concentration of NAG in the samples is then determined by comparing the O.D. of the samples to
the standrd curve.
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