Rat carcinoembryonic antigen (CEA) ELISA Kit
RatThe kit assay Rat CEA/CD66 level in the sample,
use Purified Rat CEA/CD66 to coat microtiter plate wells,
make solid-phase antibody, then add CEA/CD66 to wells,
Combined CEA/CD66 antibody which With HRP labeled,
become antibody - antigen - enzyme-antibody complex,
after washing Completely, Add TMB substrate solution,
TMB substrate becomes blue color At HRP enzyme-catalyzed,
reaction is terminated by the addition of a sulphuric acid solution
and the color change is measured spectrophotometrically at a
wavelength of 450 nm. The concentration of CEA/CD66 in the
samples is then determined by comparing the O.D. of the
samples to the standard curve.
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