Rat colon cancer-specific antigen-4 (CCSA-4) ELISA Kit
RatThe kit assay Rat CCSA-4 level in the sample,use Purified
Rat CCSA-4 antibody to coat microtiter plate wells,
make solid-phase antibody, then add CCSA-4 to wells,
Combined CCSA-4 antibody which With HRP labeled ,
become antibody - antigen - enzyme-antibody complex,
after washing Completely, Add TMB substrate solution,
TMB substrate becomes blue color At HRP enzyme-catalyzed,
reaction is terminated by the addition of a sulphuric acid solution
and the color change is measured spectrophotometrically at a
wavelength of 450 nm. The concentration of CCSA-4 in the samples
is then determined by comparing the O.D. of the samples to the
standard curve.
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