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Product name:

 Human Anti-cardiolipin antibody (ACA) ELISA Kit

Tests:

1 X 96 well plate,or 1 X 48 well plate

Sample type:

serum, plasma, culture media or any biological fluid.

Assay type:

Sandwich

Reacted Species

Human

Product overview

This ELISA kit uses Sandwich-ELISA as the method. The Microelisa stripplate provided in this kit has been pre-coated with an antigen specific to ACA. Standards or samples are added to the appropriate Microelisa stripplate wells and combined to the specific antigen. Then a Horseradish Peroxidase (HRP)-conjugated antigen specific for ACA is added to each Microelisa stripplate well and incubated. Free components are washed away. The TMB substrate solution is added to each well. Only those wells that contain ACA and HRP conjugated ACA antigen will appear blue in color and then turn yellow after the addition of the stop solution. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The presence of ACA is determined by comparing with the CUTOFF value.

Tested applications

Sandwich ELISA

Storage Instructions

The unopened kit shall be stored at [2-8 ℃] .

For opened kit can be stored at [2-8 ℃] for up to 1 month. If not be used recently, the standard should be kept in -20 ℃.