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Human Tie-1 ELISA Kit

Human
Human Tie-1 ELISA Kit
Human Tie-1 ELISA Kit

The kit assay Human Tie-1level in the sample,use Purified Human Tie-1 to coat microtiter plate wells

make solid-phase antibody, then add Tie-1 to wells, Combined Tie-1antibody which With HRP labeled , 

become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,

TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of 

a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. 

The concentration of Tie-1 in the samples is then determined by comparing the O.D. of the samples to the 

standard curve.

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11至15个工作日送达

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