Human Tie-1 ELISA Kit
HumanThe kit assay Human Tie-1level in the sample,use Purified Human Tie-1 to coat microtiter plate wells,
make solid-phase antibody, then add Tie-1 to wells, Combined Tie-1antibody which With HRP labeled ,
become antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,
TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of
a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm.
The concentration of Tie-1 in the samples is then determined by comparing the O.D. of the samples to the
standard curve.
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