Human acetylcholine (ACh) ELISA Kit
HumanThe kit assay Human ACh level in the sample,use Purified Human ACh antibody to coat microtiter plate wells,
make solid-phase antibody, then add ACh to wells, Combined ACh antibody which With HRP labeled, become
antibody - antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate
becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and
the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of ACh in the
samples is then determined by comparing the O.D. of the samples to the standard curve.
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