Human Complement 3a (C3a) ELISA Kit
HumanThe kit assay Human C3a level in the sample,use Purified Human C3a antibody to coat microtiter plate wells,
make solid-phase antibody, then add C3a to wells, Combined C3a which With HRP labeled , become antibody -
antigen - enzyme-antibody complex, after washing Completely, Add TMB substrate solution,TMB substrate becomes
blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color
change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of C3a in the samples is
then determined by comparing the O.D. of the samples to the standard curve.
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