Human NOGO-A Ab ELISA Kit
HumanThe kit assay Human NOGO-A Ab level in the sample, use Purified Human NOGO-A Ab to coat
microtiter plate wells, make solid-phase antibody, then add NOGO-A Ab to wells, Combine NOGO-A Ab
antibody with HRP labeled to form antibody-antigen -enzyme-antibody complex, after washing completely,
add TMB substrate solution,TMB substrate becomes blue color at HRP enzyme-catalyzed,
reaction is terminated by the addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm. The concentration of NOGO-A Ab in the samples is
then determined by comparing the O.D. of the samples to the standard curve.
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