Rat Free-T3 ELISA Kit
RatThe kit is for the quantitative level of Free-T3 in the sample,
adopt purified Rat Free-T3 to coat microtiter plate,
make solid-phase antibody, then add Free-T3 to wells,
Combine Free-T3 antibody with labeled HRP to form
antibody-antigen -enzyme-antibody complex, after
washing completely, add TMB substrate solution,
TMB substrate becomes blue color at HRP enzyme-catalyzed,
reaction is terminated by the addition of a stop solution and the
color change is measured at a wavelength of 450 nm.
The concentration of Free-T3 in the samples is then determined
by comparing the O.D. of the samples to the standard curve.
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