Human alcoholdehydrogenase (ADH) ELISA Kit
HumanThe kit assay Human ADH level in the sample, use Purified Human ADH to
coat microtiter plate wells, make solid-phase antibody, then add ADH to wells,
Combine ADH antibody with HRP labeled to form antibody-antigen -enzyme-antibody
complex, after washing completely, add TMB substrate solution,TMB substrate
becomes blue color at HRP enzyme-catalyzed, reaction is terminated by the
addition of a sulphuric acid solution and the color change is measured
spectrophotometrically at a wavelength of 450 nm. The concentration of
ADH in the samples is then determined by comparing the O.D. of the
samples to the standard curve.
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