Human SDF- 1βELISA Kit
HumanThe kit assay Human SDF- 1β level in the sample, use Purified Human
SDF- 1β to coat microtiter plate wells, make solid-phase antibody, then
add SDF- 1β to wells, Combine SDF- 1β antibody with HRP labeled to
form antibody-antigen -enzyme-antibody complex, after washing completely,
add TMB substrate solution,TMB substrate becomes blue color at HRP
enzyme-catalyzed, reaction is terminated by the addition of a sulphuric
acid solution and the color change is measured spectrophotometrically
at a wavelength of 450 nm. The concentration of SDF- 1β in the samples
is then determined by comparing the O.D. of the samples to the standard
curve.
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