Human Glucose-6-Phosphate Dehydrogenase(G6PD) ELISA Kit
HumanThe kit assay Human G6PD level in the sample, use Purified Human
G6PD to coat microtiter plate wells, make solid-phase antibody, then
add G6PD to wells, Combine G6PD antibody with HRP labeled to form
antibody-antigen -enzyme-antibody complex, after washing completely,
add TMB substrate solution,TMB substrate becomes blue color at HRP
ezyme-catalyzed, reaction is terminated by the addition of a sulphuric
acid solution and the color change is measured spectrophotometrically
at a wavelength of 450 nm. The concentration of G6PD in the samples is
then determined by comparing the O.D. of the samples to the standard
curve.
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